Let's suppose an abnormal protein contains three sorting signals: an ER signal sequence, an ER retention sequence, and a mitochondrial matrix-targeting sequence. The ER retention sequence is supposed to keep a protein within the ER. Where would you expect this abnormal protein to go? Note: Think carefully about the timing of events in protein sorting and which events occur cotranslationally and which occur post-translationally.
a) It would go to the ER.
b) It would go the mitochondria.
c) It would go to both the ER and mitochondria equally.
d) It would remain in the cytosol.
e) It would be secreted.
Answer: A
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