Co-immunoprecipitation (co-IP) is a common tool used by cell biologists. Below is a simplified list of steps for completing a co-IP experiment. In what order should these steps be done?
i) immobilize proteins on a membrane
ii) make a cell lysate
iii) detect the protein of interest with a labeled antibody
iv) use antibodies bound to beads to precipitate out specific protein complexes
v) separate proteins of different sizes on a gel
a) ii, v, iv, iii, i
b) ii, iv, v, i, iii
c) ii, iv, iii, v, i
d) ii, iii, v, iv, i
Answer: e) ii, v, iv, i, iii