A student set up an experiment using Drosophila melanogaster. The student wished to determine the LC-50 dose of caffeine exposure to this particular species of fruit flies. The lethal concentration is reached when 50% (LC-50) or more of the flies die when exposed to a toxin. The student mixed various concentrations of caffeine with the fruit fly media, added 50 eggs to each vial, and waited one week to analyze the results. Below are the results of the study. PICTURE
If the student were to revise this experiment, which of the following modifications would improve the experimental design of the investigation?
A) The student should run additional trials at each level of exposure.
B) The student should place additional eggs in each vial.
C) The student should use higher concentrations of caffeine.
D) The student should compare results with eggs of another insect.
Answer: A) The student should run additional trials at each level of exposure.