During which phase of the cell cycle do the chromosomes align in one plane at the center of the cell? 21:31 Biology Chapter 6 During which phase of the cell cycle do the chromosomes align in one plane at the center of the cell? a) Interphase b) Prophase c) Metaphase d) Anaphase e) G2 Answer: c) Metaphase Learn More : Share this Share on FacebookTweet on TwitterPlus on Google+