What do you predict would happen if you created a tRNA with an anticodon of 5'-CAA-3' that is charged with methionine, and added this modified tRNA to a cell-free translation system that has all the normal components required for translating RNAs?
(a) methionine would be incorporated into proteins at some positions where glutamine should be
(b) methionine would be incorporated into proteins at some positions where leucine should be
(c) methionine would be incorporated into proteins at some positions where valine should be
(d) translation would no longer be able to initiate
Answer: B