To study how proteins fold, scientists must be able to purify the protein of interest, use solvents to denature the folded protein, and observe the process of refolding at successive time points. What is the effect of the solvents used in the denaturation process?
(a) The solvents break all covalent interactions.
(b) The solvents break all noncovalent interactions.
(c) The solvents break some of the noncovalent interactions, resulting in a misfolded protein.
(d) The solvents create a new protein conformation.
Answer: B