The maximum velocity (Vmax) of an enzymatic reaction is an important piece of information regarding how the enzyme works. What series of measurements can be taken in order to infer the maximum velocity of an enzyme-catalyzed reaction?
(a) the rate of substrate consumption after the system reaches equilibrium, for several reactant concentrations
(b) the rate of product consumption shortly after mixing the enzyme and substrate
(c) the rate of substrate consumption at high levels of enzyme concentration
(d) the rate of substrate consumption shortly after mixing the enzyme and substrate, for several substrate concentrations
Answer: D