The anhydride formed between a carboxylic acid and a phosphate (Figure Q3-69A) is a high-energy intermediate for some reactions in which ATP is the energy source. Arsenate can also be incorporated into a similar high-energy intermediate in place of the phosphate (Figure Q3-69B). Figure Q3-69C shows the reaction profiles for the hydrolysis of these two high-energy intermediates. What is the effect of substituting arsenate for phosphate in this reaction?
(a) It forms a high-energy intermediate of lower energy.
(b) It forms a high-energy intermediate of the same energy.
(c) It decreases the stability of the high-energy intermediate.
(d) It increases the stability of the high-energy intermediate.
Answer: C
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