Studies conducted with a lysozyme mutant that contains an Aspa`Asn change at position 52 and a Glua`Gln change at position 35 exhibited almost a complete loss in enzymatic activity. What is the most likely explanation for the decrease in enzyme activity in the mutant?
(a) increased affinity for substrate
(b) absence of negative charges in the active site
(c) change in the active-site scaffold
(d) larger amino acids in the active site decreases the affinity for substrate
Answer: B