DNA polymerase catalyzes the joining of a nucleotide to a growing DNA strand. What prevents this enzyme from catalyzing the reverse reaction?

DNA polymerase catalyzes the joining of a nucleotide to a growing DNA strand. What prevents this enzyme from catalyzing the reverse reaction?



(a) hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi) + Pi
(b) release of PPi from the nucleotide
(c) hybridization of the new strand to the template
(d) loss of ATP as an energy source


Answer: A


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