A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut at zero, one, or two sites in a particular gene of interest.
Their next two steps, in order, should be
A) use of a fluorescent probe for the gene sequence, then electrophoresis.
B) electrophoresis of the fragments followed by autoradiography.
C) electrophoresis of the fragments, followed by the use of a probe.
D) use of a ligase that will anneal the pieces, followed by Southern blotting.
E) use of reverse transcriptase to make cDNA, followed by electrophoresis.
Answer: C